Effects of Pesticides-Laden Fruits and Vegetables on the Permeability of Renal Basement Membranes-A Route to Proteinuria
Pesticide Residues and Basement Membranes
Abstract
Objective: To study the hazardous effects of pesticide residue-laden fruits and vegetables on the permeability of renal basement membrane at proximal and distal convoluted tubules in rats.
Study Design: Observational study.
Place and Duration of Study: The study was conducted at the animal house at the Postgraduate Medical Institute (PGMI), Lahore for 21 consecutive days from 15th Nov 2018 to 6th Dec 2018.
Materials and Methods: The study design was comparative comprising of twelve Wistar male rats. The subjects were divided into two equal groups, control (group A) and experimental (group B). Body weight of subjects were noted before and after the experiment. Triazophos was given to the group B in a dose of 8.2 mg/kg body weight-sub chronic dose (1/10th of LD50) as 1:100 dilution solution by oral gavage for 21 consecutive days. Blood for serum creatinine and 24-hour urine for proteinuria were collected on day 1, 7, 14 and 21. On day 22, the subjects were sacrificed, kidneys dissected out and preserved in 10% neutral buffered formalin (NBF) for histological examination. Pre and post experiment body weight of control group was compared with experimental group.
Results: The histopathology of the renal basement membrane of group A showed continuity while group B showed interruption, vacuolization, inflammatory cell infiltration and blood vessel congestion in tissues. Biochemical analysis confirmed raised serum creatinine and proteinuria levels.
Conclusion: The pesticide residue-laden fruits and vegetables adversely affect the histology of basement membranes in proximal and distal convoluted tubules leading to disturbed renal functions. The presence of residual pesticides in the food chain is hazardous to human health.
References
Damalas CA, Eleftherohorinos IG. Pesticide exposure, safety issues, and risk assessment indicators. Int. J. Environ. Res. Pub. Health. 2011; 8: 1402-19.
Kim KH, Kabir E, Jahan SA. Exposure to pesticides and the associated human health effects. Sci. Total Environ. 2017; 575: 525-35.
Hayat K, Afzal M, Aqueel MA, Khan QM. Determination of insecticide residues in fruits, vegetables, pollen, nectar and ground water of Punjab. Pak. J. Agric. Res. (03681157). 2018; 56.
Saeed MF, Shaheen M, Ahmad I, Zakir A, Nadeem M, Chistti AA, et al. Pesticide exposure in the local community of Vehari District in Pakistan: An assessment of knowledge and residues in human blood. Sc. Total Environ. 2017; 587: 137- 44.
Farhan M, Butt ZA, Khan AU, Wahid A, Ahmad M, Kanwal A. Restoration of pesticide contaminated agricultural soil through bio-augmentation and its kinetics. Pak. J. Agric. Sci. 2017; 54.
Anwar T, Ahmad I, Tahir S. Determination of pesticide residues in soil of Nawabshah District, Sindh, Pakistan. Pak. J. Zool. 2012; 44.
Syed JH, Alamdar A, Mohammad A, Ahad K, Shabir Z, Ahmed H, et al. Pesticide residues in fruits and vegetables from Pakistan: a review of the occurrence and associated human health risks. Environ. Sci. Pollut. Res. 2014; 21: 13367-93.
Du P, Jin M, Zhang C, Chen G, Cui X, Zhang Y, et al. Highly sensitive detection of triazophos pesticide using a novel bio-bar-code amplification competitive immunoassay in a micro well plate-based platform. Sensors and Actuators B: Chemical. 2018; 256: 457-64.
Brar GS, Patyal SK, Banshtu T. Persistence of Acephate, Profenofos and Triazophos residues in brinjal fruits and soil. The Bioscan. 2017; 12: 33-7.
Shakoori A, Yazdanpanah H, Kobarfard F, Shojaee MH, Salamzadeh J. The Effects of House Cooking Process on Residue Concentrations of 41 Multi-Class Pesticides in Rice. Iran. J. Pharma. Res. IJPR. 2018; 17: 571.
Rathod GB, Parmar P, Rathod S, Parikh A. Hazards of free radicals in various aspects of health–a review. J Forensic Toxicol Pharmacol. 2014; 3: 1-7.
Sharma D, Sangha GK. Triazophos induced oxidative stress and histomorphological changes in liver and kidney of female albino rats. Pestic. Biochem. Physiol. 2014; 110: 71- 80.
Chandra MO, Raj JA, Dogra TD, Rajvanshi AC, Raina AN. Determination of median lethal dose of triazophos with DMSO in wistar rats. Asian J Pharm Clin Res. 2014; 7: 64-7.
Jain S, Mythily S, Ahmed RS, Arora VK, Banerjee BD. Induction of oxidative stress and histopathological changes
by sub-chronic doses of triazophos. Ind. J. Biochem. Biophy. 2010; 47: 388-92.
Lee G, Goosens KA. Sampling blood from the lateral tail vein of the rat. J. Vis. Exp. (JoVE). 2015; 18: 52766.
Castro BB, Colugnati FA, Cenedeze MA, Suassuna PG, Pinheiro HS. Standardization of renal function evaluation in Wistar rats (Rattus norvegicus) from the Federal University of Juiz de Fora's colony. Brazil. J. Nephrol. 2014; 36: 139-49. 17. Doeing DC, Borowicz JL, Crockett ET. Gender dimorphism in differential peripheral blood leukocyte counts in mice using cardiac, tail, foot, and saphenous vein puncture methods.
BMC clinical pathol. 2003; 3: 3.
Remuzzi A, Puntorieri S, Mazzoleni A, Remuzzi G. Sex related differences in glomerular ultrafiltration and proteinuria in Munich-Wistar rats. Kidney int. 1988; 34: 481-6.
Bancroft JD, Gamble M, editors. Theory and practice of histological techniques. Churchill Livingstone. Elsevier health sciences; 2008.
Grizzle WE, Fredenburgh JL, Myers RB. Fixation of tissues. Theory and practice of histological techniques. 2008; 6: 53-74
Khan NA, Naqvi AN, Perveen KH, Rafique MU. Lead induced nephrotoxicity with special reference to proximal tubule in albino rats. Pak. J. Pharmacol. 2008; 25: 29-35.
Rahman SA, Sattar DA. Effect of Different Concentration of Super Cyren Pesticide on Some Physiological and Histological Traits of Mice After Different Periods of Oral Administration. Iraqi J. Sci. 2018; 58: 2291-300.
Ghaffar A, Ashraf S, Hussain R, Hussain T, Shafique M, Noreen S, et al. Clinico-hematological disparities induced by triazophos (organophosphate) in Japanese quail. Pak. Vet. J. 2014; 34: 257-9.
Alt JM, Hackbarth H, Deerberg F, Stolte H. Proteinuria in rats in relation to age-dependent renal changes. Laboratory animals. 1980; 14: 95-101
